| Congresso Brasileiro de Microbiologia 2023 | Resumo: 803-2 | ||||
Resumo:Diseases caused by Staphylococcus aureus bacteria have been associated with persistent and resistant infections to usual antimicrobial therapy, so that biofilm formation is evidenced as one of the main virulence mechanisms of this pathogen. Bacteria embedded in biofilms pose risks to public health as they release free cells into their surroundings, predisposing to repeated cross-contaminations. However, studies on continuous monitoring of the biofilm formation process in Staphylococcus aureus are scarce. In this perspective, the objective of the present study was to continuously monitor the biofilm formation process using p-coumaric acid at the minimum inhibitory concentration of 2,048.0 µg/mL. P-coumaric acid was obtained commercially from Sigma-Aldrich® (USA). A clinical isolate from caprine mastitis and the strain from the American Type Culture Collection, ATCC 25923 of S. aureus, were used, which were kindly provided by the Laboratory of Microbiology and Immunology of the Federal University of Vale do São Francisco. All isolates were classified as strong biofilm producers. To investigate the biofilm inhibition effect, the crystal violet assay was used. The concentrations used were ½ and ¼ of the MIC. Optical density (OD) readings were taken at 0h, 06h, 12h, 18h, and 24h after applying the test substance to the isolates. Remarkably, the results obtained in this study show that the intensity of biofilm formation in the control groups was significantly higher than in the group treated with p-coumaric acid at concentrations of ½ and ¼ of the MIC. Changes in the kinetic parameters in the process of biofilm formation in the clinical isolate of S. aureus were observed six hours after exposure to p-coumaric acid (p<0.001). Moreover, this interference significantly prolonged throughout the 24-hour evaluation period (p<0.0001). Meanwhile, kinetic values related to ATCC 25923 only showed significant differences after 12 hours of exposure to the test substance (p<0.0001). From these results, it is possible to infer that changes in kinetic parameters did not exhibit a concentration-dependent effect of p-coumaric acid. These findings suggest the imminent potential of p-coumaric acid to disrupt the structure of S. aureus biofilms. This phenomenon can be explained by the fact that cells in the control group became sessile, while cells treated with p-coumaric acid remained in the planktonic state, indicating disruption of the exopolysaccharide architecture. In this sense, these results suggest that p-coumaric acid can be a potential therapeutic agent, as it plays a significant role in interfering with bacterial adhesion mechanisms. These findings pave the way for future therapies aimed at combating antimicrobial resistance associated with biofilm formation. Palavras-chave: Antibiofilm, Antimicrobial resistance, Biofilm kinetics, Caprine mastitis, Secondary metabolites Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |||||